Experimentation kits, ion exchange chromatography

Leverandør: G-Biosciences
BE-415
BE-415EA 4150 NOK
BE-415
Experimentation kits, ion exchange chromatography
Lærings materiell Læremateriale for kjemi Chromatography Learning Activities
Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

  • The principle ion
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