p53 transcription factor assay kit

Leverandør: Cayman Chemical

600020-96
CAYM600020-96EA 15475 NOK
CAYM600020-96
p53 transcription factor assay kit
Assay Cellulær analyse

96-Well assay for measurement of p53.


  • A sensitive, non-radioactive method of detecting p53 from whole cell lysates
  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
  • Nuclear extraction kit is available to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates


The tumor suppressor protein p53 plays a crucial role in coordinating cellular responses to genotoxic stress and holds many important clinical implications in the treatment of cancer. Cayman’s p53 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double-stranded DNA (dsDNA) sequence containing the p53 response element is immobilized onto the wells of a 96-well plate. p53 contained in a nuclear extract, binds specifically to the p53 response element and is detected by addition of a specific primary antibody directed against p53. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.


Leveringsinformasjon: Kit contains buffer, reagent A, positive control, binding buffer, primary antibody, buffer concentrate, polysorbate 20, competitor dsDNA, HRP conjugate, plate and cover sheet, developing and stop solution.

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