Restriction enzymes, SfiI, Fermentas

Leverandør: Thermo Fisher Scientific

ER1821
ER1821EA 630 NOK
ER1821
Restriction enzymes, SfiI, Fermentas
Nukleinsyre reagenser Restriction Enzymes

SfiI restriction enzyme recognizes GGCCNNNN^NGGCC sites and cuts best at 50 °C in G buffer.


  • Convenient colour-coded five buffer system
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities


Storage buffer: 10 mM Tris-HCl (pH 7,4 at 25 °C), 300 mM NaCl, 10 mM MgCl2, 1 mM DTT, 0,15% triton X-100, 0,2 mg/ml BSA and 50% (v/v) glycerol. After 50-fold overdigestion with SfiI, more than 95% of the DNA fragmentscan be ligated and recut. Minimum 5 units of theenzyme are required forcomplete digestion of 1 µg of agarose-embedded adenovirus-2 DNA in 16 hours.


Applications include molecular cloning, restriction site mapping, genotyping, southern blotting, restriction fragment length polymorphism (RFLP) and SNP.

Bestill nå

SPESIFIKASJONER

Environmentally Preferable
Applikasjon
Enzymnavn SfiI
Kilde
Overhang type 3' overhang
Kompatibel buffer FastDigest Green Buffer or FastDigest Buffer (colorless)

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