Octet® Anti-penta-HIS (HIS1K) biosensors

Leverandør: Sartorius

18-5121
SART18-5121EA 83550 NOK
SART18-5121
Octet® Anti-penta-HIS (HIS1K) biosensors
Assay Proteinanalyser

For label-free quantitation and kinetic analysis of his-tagged proteins.


  • High specificity quantitation of his-tagged proteins
  • Easy capture of his-tagged proteins for kinetic analysis with interacting protein analyte
  • High specificity and High affinity towards polyhistidine tag
  • Allows rapid analysis in purified or crude samples


The polyhistidine tag, commonly known as His-tag, is fused to recombinant proteins as a means of facilitating detection and purification. The dip and read anti-penta-HIS (HIS1K) biosensor consists of high affinity, high specificity penta-his antibody from QIAGEN pre-immobilized on a sartorius fibre optic biosensor.


In conjunction with the Octet® systems, the HIS1K biosensor provides a rapid and label-free method for his-tagged protein quantitation and kinetic analysis. The high specificity of the antibody-based biosensor enables the direct capture and quantitation of his-tagged proteins in crude lysates, column eluates, cell lysates and cell culture supernatants, serving as an alternative to traditional time consuming analytical methods such as HPLC and ELISA.

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