STEMdiff™ Branching Lung Organoid Kit

Leverandør: STEMCELL Technologies

100-0195
STMC100-0195EA 0 NOK
STMC100-0195
STEMdiff™ Branching Lung Organoid Kit
Media, Cellekultur

Serum-free media for generation and maturation of branching lung organoids from human ES and iPS cells.


STEMdiff™ Branching Lung Organoid Kit is a serum-free medium system for efficient and reproducible generation of branching lung organoids from human embryonic stem (ES) and induced pluripotent stem (iPS) cells through four stages of differentiation: 1) definitive endoderm, 2) anterior foregut endoderm, 3) lung bud organoid, and 4) branching lung organoids. STEMdiff™ Branching Lung Organoid Kit has been optimised for differentiation of cells maintained in mTeSR™1. Resulting organoids will develop proximal and distal-like branching airway epithelial structures expressing EPCAM, NKX2.1, SOX2, SOX9, MUC1, and P63.


When maintained beyond 28 days in a Matrigel® sandwich, there is an increase in expression levels of markers for more mature lung cells, such as SFTPC, SFTPB, and ABCA3. For extended periods of organoid culture (>28 days in the Matrigel® sandwich), the components required for branching lung organoid maturation are available in STEMdiff™ Branching Lung Organoid Maturation Kit.


NB: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').

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