Artikkenummer: (IMMRIR1015)

Produsent: ImmunoReagents

Beskrivelse: ImmunoReagents Sheep IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Sheep IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

UOM: 1 * 2 mL

Tilbud

Produsent: G-Biosciences

Beskrivelse: Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

Artikkenummer: (TCIAP2461-1VIAL)

Produsent: TCI

Beskrivelse: Protein A is a type I membrane protein produced by several strains of Staphylococcus aureus. It has high-affinity binding sites that enable the purification of IgG obtained from various species such as humans, rabbit, mouse, and bovine. Protein A Agarose is prepared using a covalent coupling method that maintains the stability and binding characteristics of the product.
By using our resin, human IgGs can be eluted under milder conditions (such as at pH 4.0) as compared to elution by conventional methods.
Additional information is available on our website.

UOM: 1 * 1 Each

Tilbud

Artikkenummer: (IMMRIR1010)

Produsent: ImmunoReagents

Beskrivelse: ImmunoReagents Goat IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Goat IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

UOM: 1 * 2 mL

Tilbud

Artikkenummer: (TCIAR0235-1VIAL)

Produsent: TCI

Beskrivelse: The rPSL1a is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Polyporus squamosus. This lectin preferably binds to a [Siaα(2-6)Gal] glycan epitope (It very slightly interacts with lactose.).
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rPSL1a-LecBeads (1 ml gel) shows a binding capacity of about 10 mg or more human Transferrin [hTF] through its binding to a terminal [Siaα(2-6)Gal] glycan epitope on hTF.

UOM: 1 * 1 Each

Tilbud

Produsent: G-Biosciences

Beskrivelse: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Artikkenummer: (786-802)

Produsent: G-Biosciences

Beskrivelse: The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

UOM: 1 * 1 Each

Tilbud

Produsent: Genscript

Beskrivelse: Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.

Artikkenummer: (786-803)

Produsent: G-Biosciences

Beskrivelse: The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

UOM: 1 * 1 Each

Tilbud

Produsent: G-Biosciences

Beskrivelse: Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

Produsent: G-Biosciences

Beskrivelse: This avidin resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labelled antibodies.

Produsent: G-Biosciences

Beskrivelse: Cobalt chelating resin, 3 ml columns, for the purification of 6X His proteins

Produsent: G-Biosciences

Beskrivelse: Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Artikkenummer: (IMMRIR1013)

Produsent: ImmunoReagents

Beskrivelse: ImmunoReagents Mouse IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Mouse IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

UOM: 1 * 2 mL

Tilbud

Produsent: G-Biosciences

Beskrivelse: Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Produsent: G-Biosciences

Beskrivelse: Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, <i>E.coli</i>, yeast, plant or as a control set that contains one 2 mg vial of each.