Du søkte etter: Western Blotting Reagents

Artikkenummer: (A3417.5000)

Produsent: PanReac AppliChem

Beskrivelse: The CheLuminate-HRP PicoDetect is a complete kit with ready to use reagents for chemiluminescent detection of immobilised proteins (Western blot) or immobilised nucleic acids (Southern or Northern blot), conjugated with horseradish peroxidase (HRP) directly or indirectly.

UOM: 1 * 1 Each

Tilbud

Produsent: Rockland Immunochemicals

Beskrivelse: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Produsent: Cytiva

Beskrivelse: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Artikkenummer: (RPN3006)

Produsent: Cytiva

Beskrivelse: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

UOM: 1 * 1 Items

Tilbud

Produsent: Cytiva

Beskrivelse: AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Produsent: Cytiva

Beskrivelse: Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

HMS Sertifikater

Produsent: Cytiva

Beskrivelse: Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Produsent: Cytiva

Beskrivelse: Chemiluminescence detection reagent for detection of high to medium abundant proteins.

Artikkenummer: (RPN5785)

Produsent: Cytiva

Beskrivelse: ECF™ substrate for Western blotting

UOM: 1 * 1 Items

Tilbud

Artikkenummer: (RPN3005)

Produsent: Cytiva

Beskrivelse: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

UOM: 1 * 1 Items

Tilbud

Produsent: Cytiva

Beskrivelse: CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Artikkenummer: (RPN2235)

Produsent: Cytiva

Beskrivelse: Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

UOM: 1 * 1 Each

HMS Sertifikater Tilbud

Produsent: Cytiva

Beskrivelse: Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Produsent: Cytiva

Beskrivelse: Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

HMS Sertifikater

Artikkenummer: (RPN2125)

Produsent: Cytiva

Beskrivelse: Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents

UOM: 1 * 40 g

Tilbud

Artikkenummer: (ICNA08980641)

Produsent: MP Biomedicals

Beskrivelse: This antibody diluent is compatible with common stabilising compounds at pH 7,3.

UOM: 1 * 125 mL

Tilbud