VWR®, Taq DNA polymerase glycerol free

Leverandør: VWR Chemicals

733-1817EA 3300 NOK
733-1817 733-1999 733-2038 733-2406 733-3843
VWR®, Taq DNA polymerase glycerol free
Nukleinsyre reagenser End-point PCR Enzymes and Kits

VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.


  • Most suitable choice for routine applications where high fidelity is not required
  • High performance, thermostable DNA polymerase
  • Optimal for TA cloning


VWR® Taq DNA Polymerase Glycerol Free has both a 5'→3' DNA polymerase and a 5'→3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity (no proofreading ability). VWR® Taq DNA Polymerase Glycerol Free leaves an A' overhang, which makes the enzyme ideal for TA cloning.


10X Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween-20®


10X Extra Buffer: 100 mM Tris-HCL pH 8,3, 500 mM KCl, 15 mM MgCl₂, 1% Triton X-100


10X Mg-Free Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 1% Tween-20®


10X Mg-Free Extra Buffer: Tris-HCL pH 8,3, KCl, 1% Triton X-100

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SPESIFIKASJONER

Beskrivelse Taq DNA Polymerase Glycerol Free 5U/µl 1.000 units Taq DNA Polymerase Glycerol Free 5U/µl 5.000 units Taq DNA Polymerase Glycerol Free 5U/µl 2.000.000 units Taq DNA Polymerase Glycerol Free 5U/µl 100.000 units Taq DNA Polymerase Glycerol Free 5U/µl 375.000 units
Environmentally Preferable
Format Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer
Egenskaper Glycerol free Glycerol free Glycerol free Glycerol free Glycerol free
Varmstart No No No No No
Konsentrasjon 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl
Enheter 1000 5000 2000000 100000 375000
Størrelse 2 x 0.1 ml + 6 x 1.5 ml 10 x 0.1 ml Bulk 20 x 1 ml 1 x 75 ml
Bufersammensetning 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2 25 mM MgCl2 No buffer No buffer No buffer
Høy troskap Nei Nei Nei Nei Nei
amplicon størrelse ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb
Processivity Standard Standard Standard Standard Standard
Eksonukleaseaktivitet 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’
Product overhang 3'A 3'A 3'A 3'A 3'A
Lagringsforhold – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Applikasjon Freeze-drying,Standard amplification Freeze-drying,Standard amplification Freeze-drying,Standard amplification Freeze-drying,Standard amplification Freeze-drying,Standard amplification

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