DNA methylation ELISA kit

Leverandør: Cayman Chemical

589324-96S 589324-96 589324-480S 589324-480
CAYM589324-96SEA 14350 NOK
CAYM589324-96S CAYM589324-96 CAYM589324-480S CAYM589324-480
DNA methylation ELISA kit
Assay ELISAs

A competitive assay that can be used for the quantification of 5-methyl-2'-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices.


  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 5-methyl-2'-deoxy cytidine levels down to three ng/ml
  • Read: Colorimetric at 405 to 420 nm


DNA methylation is an important epigenetic process regulating gene expression. Methylation occurs on carbon 5 of 2'-deoxy-cytidine yielding the modified base 5-methyl-2'-deoxy cytidine. The methylation pattern of cells is tightly regulated during development with the methylation profile being transmitted from parent to daughter cells during cell division.


Methylation results in long-term silencing of genes, while unmethylated regions of DNA can be actively transcribed. Global changes in methylation can be quantified by measuring plasma or urinary levels of 5-methyl-2'-deoxy cytidine. These changes in methylation can provide valuable information about cancer status of an individual. For example, patients with leukemia excrete significantly elevated levels of 5-methyl-2'-deoxy cytidine compared to healthy individuals.


Global methylation within tissues can be measured in a similar manner, allowing study of tissue-specific changes that occur as a result of differentiation, aging, or carcinogenesis. Cayman’s DNA Methylation ELISA Kit is a competitive assay that can be used for the quantification of 5-methyl-2'-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices.


Forpakning: Kit contains antibody, tracer, standard, ELISA and wash buffer concentrate, polysorbate 20, plate and cover, ellman's reagent, tracer and antiserum dye.

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SPESIFIKASJONER

Environmentally Preferable
Primary antibody reactivity
Vert
Sensitivitet ~3 ng/ml (80% B/B₀)
Påvisningsspekter 1,17 - 150 ng/ml
Mid point ~8 - 15 ng/ml (50% B/B₀)
Utviklingstid 90 - 120 min
Inkuberingstid 18 hr
Lagringstemp –20 °C

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