TEMPase Hot Start DNA Polymerase

Leverandør: VWR Chemicals

733-1331EA 2025 NOK
733-1331 733-1333 733-1838 733-2556 733-2553 733-2552 733-2559
TEMPase Hot Start DNA Polymerase
Nukleinsyre reagenser End-point PCR Enzymes and Kits

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR. A glycerol-free TEMPase Hot Start DNA Polymerase is also available for automation and freeze drying.


The GC-Rich Template kit is specifically designed for difficult GC-rich sequences. Combined with TEMPase, GC buffers I and II promote excellent amplification. The kit is designed for initial testing before using one of the GC-TEMPase 2X Master Mixes.


Leveringsinformasjon: VWR® TEMPase DNA polymerases generally include two different buffers, Key Buffer and Combination Buffer, which are each suited for different PCR requirements. Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) and minimises the need for optimisation of the Mg²⁺ concentration or the annealing temperature in most primer-template systems. Combination Buffer is a mixture of K+ and NH⁴⁺. It combines high specificity with good product yield and high tolerance to optimisation of primer annealing temperatures and Mg²⁺ concentrations due to its balanced ammonium-potassium formulation. Each buffer contains 15 mM MgCl? (1,5 mM in final volume). Additional MgCl? for easy optimisation is included in a separate vial.

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SPESIFIKASJONER

Beskrivelse TEMPase HotStart DNA Polymerase 5U/µl 500 units TEMPase HotStart DNA Polymerase 5U/µl 2500 units TEMPase HotStart DNA Polymerase 5U/µl 10.000 units TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 500 units TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units
Environmentally Preferable
Format Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer
Egenskaper Glycerol free Glycerol free Glycerol free Glycerol free
Varmstart Chemically modified Chemically modified Chemically modified Chemically modified Chemically modified Chemically modified Chemically modified
Konsentrasjon 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl
Enheter 500 2500 10000 2500 2500 500 2500
Størrelse 1 x 0.1 ml + 3 x 1.5 ml 5 x 0.1 ml + 15 x 1.5 ml 3 x 0.667 ml + 18 x 5 ml 5 x 0.1 ml + 10 x 1.5 ml 5 x 0.1 ml + 5 x 1.5 ml 1 x 0.1 ml + 1 x 1.5 ml 5 x 0.1 ml + 10 x 1.5 ml
Bufersammensetning 10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2 10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2 10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2 10x Key Buffer and 25 mM MgCl2 25 mM MgCl2 25 mM MgCl2 10x Combination Buffer and 25 mM MgCl2
Høy troskap Nei Nei Nei Nei Nei Nei Nei
amplicon størrelse ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb
Processivity Standard Standard Standard Standard Standard Standard Standard
Eksonukleaseaktivitet 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’
Product overhang 3'A 3'A 3'A 3'A 3'A 3'A 3'A
Lagringsforhold – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Applikasjon Multiplex Multiplex Multiplex Freeze-drying,Standard amplification Freeze-drying,Standard amplification Freeze-drying,Standard amplification Freeze-drying,Standard amplification

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