VWR®, TEMPase Hot Start 2× master mix

Leverandør: VWR Chemicals

733-1841EA 31450 NOK
733-1841 733-1840 733-2569 733-2582 733-2585
VWR®, TEMPase Hot Start 2× master mix
Nukleinsyre reagenser End-point PCR Enzymes and Kits

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.


The blue loading dye in Blue TEMPase Hot Start DNA Polymerase Master Mix facilitates direct gel loading, and eliminates the need for separate loading dye - no need for time-consuming sample preparation before electrophoresis.


Multiplex 2× Master Mix is composed of TEMPase Hot Start DNA Polymerase and a specialised buffer system designed for multiplex PCR.


Leveringsinformasjon: TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are available in two variations, either based on Key Buffer (Master Mix K) or Combination Buffer (Master Mix C) to suit different PCR requirements. Additional MgCl₂ is included in the kit to enable optimisation.

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SPESIFIKASJONER

Beskrivelse Blue TEMPase Hot Start DNA Polymerase 2X Master Mix C
1.5 mM MgCl2 2500 reactions
TEMPase Hot Start DNA Polymerase 2X Master Mix C
1.5 mM MgCl2 2500 reactions
Multiplex TEMPase 2X Master Mix
1.5 mM MgCl2 2500 reactions
TEMPase Hot Start DNA Polymerase 2X Master Mix A
1.5 mM MgCl2 2500 reactions
Blue TEMPase Hot Start DNA Polymerase 2X Master Mix A
1.5 mM MgCl2 2500 reactions
Environmentally Preferable
Format Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs
Egenskaper Direct loading Direct loading
Varmstart Chemically modified Chemically modified Chemically modified Chemically modified Chemically modified
Konsentrasjon 2x 2x 2x 2x 2x
Enheter 2500 r Mix 2500 2500 r Mix 2500 2500 r Mix 2500 2500 r Mix 2500 2500 r Mix 2500
Størrelse 50 x 1.25 ml 50 x 1.25 ml 50 x 1.25 ml + 3 x 1.5 ml 50 x 1.25 ml 50 x 1.25 ml
Bufersammensetning Based on Combination buffer Based on Combination buffer 25 mM MgCl2 Based on Ammonium buffer Based on Ammonium buffer
Høy troskap Nei Nei Nei Nei Nei
amplicon størrelse ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb
Processivity Standard Standard Standard Standard Standard
Eksonukleaseaktivitet 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’
Product overhang 3'A 3'A 3'A 3'A 3'A
Lagringsforhold – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Applikasjon Multiplex Multiplex Multiplex Multiplex Multiplex

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