VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix

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733-2567EA 2050 NOK
733-2567 733-2562 733-2561 733-2565
VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix
Nukleinsyre reagenser End-point PCR Enzymes and Kits

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.


The GC-Rich Template kit is specifically designed for difficult GC-rich sequences. Combined with TEMPase, GC buffers I and II promote excellent amplification. The kit is designed for initial testing before using one of the GC-TEMPase 2X Master Mixes.


Ready to use GC TEMPase 2× Master Mixes I and II are designed for amplification of GC-rich sequences.

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SPESIFIKASJONER

Beskrivelse GC-rich DNA Target Kit
TEMPase HotStart DNA Polymerase 5U/µl 500 units
GC TEMPase 2X Master Mix I
1.5 mM MgCl2 2500 reactions
GC TEMPase 2X Master Mix I
1.5 mM MgCl2 500 reactions
GC TEMPase 2X Master Mix II
1.5 mM MgCl2 2500 reactions
Environmentally Preferable
Format Enzyme with/without buffer Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs
Egenskaper GC-rich GC-rich GC-rich
Varmstart Chemically modified Chemically modified Chemically modified Chemically modified
Konsentrasjon 5 U/µl 2x 2x 2x
Enheter 500 2500 r Mix 2500 500 r Mix 500 2500 r Mix 2500
Størrelse 1 x 0.1 ml + 3 x 1.5 ml 50 x 1.25 ml 10 x 1.25 ml 50 x 1.25 ml
Bufersammensetning 4x GC Buffer I, 4 x GC Buffer II and 25 mM MgCl2 Based on GC buffer I Based on GC buffer I Based on GC buffer II
Høy troskap Nei Nei Nei Nei
amplicon størrelse ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb
Processivity Standard Standard Standard Standard
Eksonukleaseaktivitet 5’→3’ 5’→3’ 5’→3’ 5’→3’
Product overhang 3'A 3'A 3'A 3'A
Lagringsforhold – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Applikasjon Multiplex Standard amplification,GC-rich template Standard amplification,GC-rich template Standard amplification,GC-rich template

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