Lugol's iodine solution, Sigma-Aldrich®

Leverandør: Merck


M-Clarity™ quality level = MQ300

1.09261.1000 1.09261.2500
1.09261.1000EA 474 NOK
1.09261.1000 1.09261.2500
Lugol's iodine solution, Sigma-Aldrich®
Lugol's iodine solution

Diluted iodine-potassium iodide solution used to form a crystal violet dye-iodine complex in Gram staining.


In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with Gram's iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A 500 mL PE-bottle of Gram's Lugol's solution is sufficient for staining up to 250 slides. Lugol’s solution is also a part of the Gram-color stain set. It is an IVD & CE product.

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Spesifikasjon Testresultater

Suitability for microscopy passes test
gram-positve microorganisms blue-violet
gram-negative microorganisms pink to red

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