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Du søkte etter: Cloning+Kits+and+Ligation


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Product Image-K1232

PCR cloning kit, CloneJET

Produsent: Thermo Fisher Scientific
Beskrivelse: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-EL0016

T4 DNA ligase

Artikkenummer: (EL0016)
Produsent: Thermo Fisher Scientific
Beskrivelse: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
UOM: 1 * 1 Each

Sertifikater


Image Unavailable-70573-3

Ligation premix, Clonables™

Produsent: Merck Millipore (Novagen)
Beskrivelse: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Product Image-K1261

LIC cloning and expression kits, aLICator™, Fermentas

Produsent: Thermo Fisher Scientific
Beskrivelse: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Product Image-GA1100-50

DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

HMS

Product Image-SYGEGA1100-4X50

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Product Image-27-0361-01

T4 DNA ligase

Artikkenummer: (27-0361-01)
Produsent: Cytiva
Beskrivelse: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
UOM: 1 * 1 Each
Product Image-GA1200-10

Gibson Assembly® Ultra kit

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

HMS

Product Image-OXNTSQK-LSK114-XL

Ligation Sequencing Kits

Produsent: Oxford Nanopore Technologies
Beskrivelse: Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.

Sertifikater

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T7Select® phage display system, Novagen®

Produsent: Merck Millipore (Novagen)
Beskrivelse: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Product Image-GA1100-10MM

Gibson Assembly® HiFi 1 Step, Master mixes

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

HMS

Product Image-GA1200-50MM

Gibson Assembly® Ultra, Master mixes

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

HMS

Image Unavailable-70595-3

pSTBlue-1 AccepTor™ vector kit

Produsent: Merck Millipore (Novagen)
Beskrivelse: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Product Image-EDVO300

Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Artikkenummer: (EDVO300)
Produsent: EDVOTEK
Beskrivelse: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.
UOM: 1 * 1 Each
Image Unavailable-ENZOALX8049120100

Anti-TRAIL-R1 Mouse Monoclonal Antibody [clone: DJR1]

Artikkenummer: (ENZOALX8049120100)
Produsent: ENZO LIFE SCIENCES
Beskrivelse: DR4 is 56kDa member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261. It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.
UOM: 1 * 1 Each
Image Unavailable-ENZOALX8049080100

Anti-TRAIL-R3 Mouse Monoclonal Antibody [clone: DJR3]

Artikkenummer: (ENZOALX8049080100)
Produsent: ENZO LIFE SCIENCES
Beskrivelse: DcR1 is a 65kDa GPI-anchored member of the TNFR superfamily (TNFRSF10C) also known as TRAIL-R3, TRID, CD263, and LIT. Although not expressed by most tumors, DcR1 is expressed at low levels on normal tissues, acting as a TRAIL decoy receptor, thus inhibiting apoptosis. DcR1 is termed a decoy receptor, as it does not have a functional death domain to induce apoptosis following TRAIL ligation.
UOM: 1 * 1 Each
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Lager for dette punktet er begrenset, men kan være tilgjengelig i en lagerbygning i nærheten av deg. Vennligst sørg for at du er logget inn på nettstedet, slik at tilgjengelige lager kan vises. Hvis call er fortsatt vises og du trenger hjelp, kan du ringe oss på 1-800-932 - 5000.
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NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
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