Du søkte etter: Merck Millipore (Novagen)

Produsent: Merck Millipore (Novagen)

Beskrivelse: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Produsent: Merck Millipore (Novagen)

Beskrivelse: Pellet Paint® NF Co-Precipitant er et ikke-fluorescerende carrier-molekyl som er kompatibel med fluoreserende sekvensering. Den bidrar til at du enkelt kan fjerne BigDye®-terminatorer under alkoholutfellingen fra sekvenseringsreaksjonsproduktene. Sekvenseringsreaksjoner kan raskt felles ut med sentrifugeringstider på 10 minutter. Det lett synlige carrier-molekylet er en bekreftelse på at utfellingen har skjedd. Sekvenseringsproduktene pelleteres effektivt, og fargemerkede terminatorer blir værende igjen i supernantanen under alkoholutfellingen ved hjelp av utfellingsprotokollene fra Applied Biosystems. Resuspendering av pelleterte sekvenseringsprodukter i deionisert formamid kan bekreftes ved å kontrollere at carrier er oppløst.

Produsent: Merck Millipore (Novagen)

Beskrivelse: Perfect DNA™-ladders inneholder DNA med praktiske størrelser som er enkle å huske, for agarosegelanalyse. Markørene har ensartede båndintensiteter og dekker et bredt område av DNA-størrelser.

HMS

Produsent: Merck Millipore (Novagen)

Beskrivelse: KOD HiFi DNA-polymerase er et unikt proofreadingsenzym, som er isolert fra den ekstremt termofile <i>Thermococcus kodakaraensis</i> KOD1, som har en overlegen prosessivitet og fidelitet som gjør det mulig med raskere og mer nøyaktig PCR-amplifisering enn det som kan oppnås med tradisjonelle enzymer.

Artikkenummer: (71840-3)

Produsent: Merck Millipore (Novagen)

Beskrivelse: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

UOM: 1 * 125 µl

Tilbud

Produsent: Merck Millipore (Novagen)

Beskrivelse: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.

HMS Sertifikater

Produsent: Merck Millipore (Novagen)

Beskrivelse: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Artikkenummer: (71978-3)

Produsent: Merck Millipore (Novagen)

Beskrivelse: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 Each

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Artikkenummer: (69831-3)

Produsent: Merck Millipore (Novagen)

Beskrivelse: The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.

UOM: 1 * 1 Each

Tilbud

Produsent: Merck Millipore (Novagen)

Beskrivelse: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Produsent: Merck Millipore (Novagen)

Beskrivelse: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Produsent: Merck Millipore (Novagen)

Beskrivelse: D-Tube™ dialyser, Mega, for D-Tube™ dialyzers

Artikkenummer: (69660-3)

Produsent: Merck Millipore (Novagen)

Beskrivelse: The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

UOM: 1 * 1 Each

Tilbud

Produsent: Merck Millipore (Novagen)

Beskrivelse: Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

Artikkenummer: (70852-3)

Produsent: Merck Millipore (Novagen)

Beskrivelse: The SpinPrep™ Gel DNA Kit enables efficient extraction of DNA fragments of 150 bp to >12000 bp in size from agarose gels. The procedure uses GelMelt™ solution to dissolve the gel slice, followed by adsorption of the DNA to a silica membrane in a spin column format. After a wash step, the purified DNA is eluted in low salt buffer. Each spin column can bind up to 20 µg DNA. Recoveries are consistently in the range of 50 to 90% under standard conditions.

UOM: 1 * 1 Each

Tilbud

Artikkenummer: (69203-3)

Produsent: Merck Millipore (Novagen)

Beskrivelse: Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.

UOM: 1 * 5 mL

Tilbud