Pellet Paint® Co-Precipitant
Leverandør: Merck Millipore (Novagen)
Pellet Paint® Co-Precipitant er et, farget carriermolekyl utarbeidet for bruk ved alkoholutfelleing av nukleinsyrer. Reaksjonen tar 2 minutter og krever ikke inkubasjon ved lav temperatur eller forlenget sentrifugering. Både RNA og DNA presipiteres effektivt, også i de mest fortynnede løsninger (2 ng/ml) og pelleten med den rosa fargen spores enkelt. Pelleten kan enkelt følges under vaskeprosedyren, noe som hindrer tap under håndtering og sikrer at pelleten resuspenderes.
- Suitable for the rapid, quantitative precipitation of DNA and RNA, including PCR clean-up and template preparation
- Compatible with most molecular biology procedures and free of contaminating nucleic acids and nucleolytic enzymes
- Although Pellet Paint absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible using the absorbance ratio provided as a correction factor when determining nucleic acid concentration
- Compatible with Cy5®-based automated sequencers
Most PCR applications benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated. Pellet Paint Co-Precipitant is ideal for this purpose because the procedure is rapid, primers <50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely re-suspended following the precipitation step.
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