Du søkte etter: Reagenser for proteinekstraksjon

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Artikkenummer: (786-244)
Produsent: G-Biosciences
Beskrivelse: SilverOUT removes interfering silver ions from gel pieces.
UOM: 1 * 1 Each


Artikkenummer: (786-681)
Produsent: G-Biosciences
Beskrivelse: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
UOM: 1 * 1 Each


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Artikkenummer: (786-793)
Produsent: G-Biosciences
Beskrivelse: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
UOM: 1 * 5 mL


Artikkenummer: (786-598)
Produsent: G-Biosciences
Beskrivelse: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
UOM: 1 * 5 mL


Produsent: ENZO LIFE SCIENCES
Beskrivelse: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

New Product

Artikkenummer: (DGA01)
Produsent: G-Biosciences
Beskrivelse: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 Items


Artikkenummer: (786-241)
Produsent: G-Biosciences
Beskrivelse: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
UOM: 1 * 1 Each


Produsent: G-Biosciences
Beskrivelse: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Artikkenummer: (786-599)
Produsent: G-Biosciences
Beskrivelse: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL


Artikkenummer: (PIER90054)
Produsent: Thermo Fisher Scientific
Beskrivelse: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 1 Each

HMS


Artikkenummer: (PIER90056)
Produsent: Thermo Fisher Scientific
Beskrivelse: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM: 1 * 1 Each


Artikkenummer: (PIER90053)
Produsent: Thermo Fisher Scientific
Beskrivelse: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 1 Each

HMS


Artikkenummer: (ENZOBMLKI1761250)
Produsent: ENZO LIFE SCIENCES
Beskrivelse: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL

New Product


Produsent: Thermo Fisher Scientific
Beskrivelse: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Artikkenummer: (ENZOBMLKI1050300)
Produsent: ENZO LIFE SCIENCES
Beskrivelse: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM: 1 * 300 µl

New Product


Artikkenummer: (PIER90067)
Produsent: Thermo Fisher Scientific
Beskrivelse: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
UOM: 1 * 1 Each


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Lager for dette punktet er begrenset, men kan være tilgjengelig i en lagerbygning i nærheten av deg. Vennligst sørg for at du er logget inn på nettstedet, slik at tilgjengelige lager kan vises. Hvis call er fortsatt vises og du trenger hjelp, kan du ringe oss på 1-800-932 - 5000.
NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
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