Du søkte etter: End-point PCR Enzymes and Kits

Produsent: FINNZYMES REAGENTS

Beskrivelse: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Produsent: FINNZYMES REAGENTS

Beskrivelse: Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.

Produsent: FINNZYMES REAGENTS

Beskrivelse: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Produsent: MP Biomedicals

Beskrivelse: <i>Taq</i> DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium <i>Thermus aquaticus</i>. The recombinant form of this enzyme is expressed in <i>E. coli</i>. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

Produsent: MP Biomedicals

Beskrivelse: Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant <i>Taq</i> DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Artikkenummer: (71975-3)

Produsent: Merck Millipore (Novagen)

Beskrivelse: KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

UOM: 1 * 200 AE

Tilbud

Artikkenummer: (71007-3)

Produsent: Merck Millipore (Novagen)

Beskrivelse: NovaTaq PCR Master Mix er en 2X konsentrert ferdig blanding av NovaTaq DNA Polymerase, ultrarene deoksynukleotider, og reaksjonsbuffer uten magnesiumklorid. Master Mix forenkler oppsettet av PCR-reaksjoner og gir fordeler i form av tidsbesparing, konsistens og minimal fare for kontaminering. Det er bare å tilsette NovaTaq PCR Master Mix til et like stort volum som inneholder den nødvendige mengden magnesiumklorid, DNA-templet, og primere, og så er reaksjonen klar for PCR-kjøring. Den endelige reaksjonen inneholder 2,5 enheter av NovaTaq DNA-polymerase per 100 µl. Løsningen rekker til 200 standard 50 µl (eller 100×100 µl) PCR-reaksjoner.

UOM: 1 * 250 EE

Tilbud

Produsent: MP Biomedicals

Beskrivelse: The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide <i>Taq</i> DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

Produsent: G-Biosciences

Beskrivelse: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Artikkenummer: (ICNA11EPTAG100)

Produsent: MP Biomedicals

Beskrivelse: Taq-&GO™ is a ready to use optimised reaction mix for PCR.

UOM: 1 * 1 mL

Tilbud

Produsent: G-Biosciences

Beskrivelse: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Produsent: Merck Millipore (Novagen)

Beskrivelse: KOD HiFi DNA-polymerase er et unikt proofreadingsenzym, som er isolert fra den ekstremt termofile <i>Thermococcus kodakaraensis</i> KOD1, som har en overlegen prosessivitet og fidelitet som gjør det mulig med raskere og mer nøyaktig PCR-amplifisering enn det som kan oppnås med tradisjonelle enzymer.

Produsent: Cytiva

Beskrivelse: For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

Artikkenummer: (25-1500-01)

Produsent: Cytiva

Beskrivelse: illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

UOM: 1 * 1 Each

Tilbud

Produsent: Cytiva

Beskrivelse: PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

Produsent: Cytiva

Beskrivelse: illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.