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STEMdiff™ Branching Lung Organoid Kit

Artikkenummer: (STMC100-0195)

Produsent: STEMCELL Technologies

Beskrivelse: Serum-free media for generation and maturation of branching lung organoids from human ES and iPS cells.

UOM: 1 * 1 Each

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7-Aminoactinomycin D (7-AAD) cell viability stain

Produsent: STEMCELL Technologies

Beskrivelse: 7‐AAD (7‐Aminoactinomycin D) is a fluorescent cell viability dye which is excluded from live cells with intact membranes but penetrates dead or damaged cells and binds to double‐stranded DNA with high affinity by intercalating between GC base pairs.

Conditional Reprogramming (CR) Medium

Artikkenummer: (STMC100-0352)

Produsent: STEMCELL Technologies

Beskrivelse: Conditional reprogramming medium for epithelial cell expansion.

UOM: 1 * 250 mL

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Mouse Recombinant TNF-alpha

Produsent: STEMCELL Technologies

Beskrivelse: Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that activates NF-κB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancer cells in vitro by stimulating anti-tumor immunosuppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová andamp; Hosek). Other effects of TNF-α include vasodilatation and edema formation.

Human Recombinant GRO-beta (CXCL2)

Produsent: STEMCELL Technologies

Beskrivelse: GRO (growth-regulated oncogene)-beta or CXCL2 is a member of the CXC family, which plays an integral role in recruitment and activation of neutrophils and basophils in response to tissue injury and microbial infection. GRO-beta and GRO-gamma are closely related to GRO-alpha and share 90% and 86% amino acid sequence homology, respectively, with GRO-alpha. Receptor-binding studies have demonstrated that GRO-alpha, -beta, and -gamma signal mainly through G protein-coupled receptors CXCR1 and CXCR2 (Ahuja and Murphy). GRO-beta is expressed in epithelial cells, monocytes, fibroblasts, and melanocytes and is further induced during inflammatory, epithelialization, and angiogenic processes, for example during the wound healing process of human burn wounds (Zaja-Milatovic and Richmond). GRO-beta also stimulates mitogenesis in certain human melanoma cells (Unemori <i>et al.</i>).

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