Du søkte etter: Buffere, ekstrasjons

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Artikkenummer: (786-411)
Produsent: G-Biosciences
Beskrivelse: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
UOM: 1 * 250 mL


Produsent: Thermo Fisher Scientific
Beskrivelse: This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Produsent: Thermo Fisher Scientific
Beskrivelse: This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Produsent: OMEGA BIO-TEK
Beskrivelse: TL buffer, Omega Bio-Tek

HMS

Produsent: G-Biosciences
Beskrivelse: FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Produsent: Thermo Fisher Scientific
Beskrivelse: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Produsent: Thermo Fisher Scientific
Beskrivelse: The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Produsent: Thermo Fisher Scientific
Beskrivelse: HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
Artikkenummer: (J60132.AK)
Produsent: Thermo Fisher Scientific
Beskrivelse: Denaturing lysis buffer, Thermo Fisher Scientific
UOM: 1 * 250 mL

HMS Sertifikater


Produsent: Thermo Fisher Scientific
Beskrivelse: RBC lysis buffer for mouse, Thermo Fisher Scientific
Produsent: Merck Millipore (Novagen)
Beskrivelse: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Produsent: Thermo Fisher Scientific
Beskrivelse: For cell lysis and protein extraction.
Produsent: Thermo Fisher Scientific
Beskrivelse: NP-40 lysis buffer, Thermo Fisher Scientific
Produsent: Thermo Fisher Scientific
Beskrivelse: Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific
Artikkenummer: (J60143.AK)
Produsent: Thermo Fisher Scientific
Beskrivelse: NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific
UOM: 1 * 250 mL

HMS Sertifikater


Artikkenummer: (ROCKMB-078-0015)
Produsent: Rockland Immunochemicals
Beskrivelse: Cell lysis buffer is used to lyse cells under non-denaturing conditions.
UOM: 1 * 15 mL


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Lager for dette punktet er begrenset, men kan være tilgjengelig i en lagerbygning i nærheten av deg. Vennligst sørg for at du er logget inn på nettstedet, slik at tilgjengelige lager kan vises. Hvis call er fortsatt vises og du trenger hjelp, kan du ringe oss på 1-800-932 - 5000.
NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
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Produkt (er) merket med dette symbolet er ikke lenger tilgjengelig og selges til lageret er tomt. Alternativer kan være tilgjengelige ved å søke med VWR Katalognummer ovenfor. Hvis du trenger ytterligere hjelp, ring VWR kundeservice 22900000
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