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qPCR assays are used for many applications, including gene expression analysis, microRNA analysis, single nucleotide polymorphism, genotyping, copy number variation analysis, and even protein analysis. These assays are easy to implement and can be employed for both relative and absolute quantification methods. Successful qPCR assays require efficient and specific amplification of the product. A range of sample volumes can be tested simultaneously, with options to test multiple well-plates simultaneously in some models.