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Ligation premix, Clonables™

Produsent: Merck Millipore (Novagen)
Beskrivelse: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Product Image-K1232

PCR cloning kit, CloneJET

Produsent: Thermo Fisher Scientific
Beskrivelse: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

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Consistency-Accuracy-Speed-Efficiency

C.A.S.E.
Product Image-EL0016

T4 DNA ligase

Artikkenummer: (EL0016)
Produsent: Thermo Fisher Scientific
Beskrivelse: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
UOM: 1 * 1 Each

Sertifikater


Product Image-27-0361-01

T4 DNA ligase

Artikkenummer: (27-0361-01)
Produsent: Cytiva
Beskrivelse: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
UOM: 1 * 1 Each
Product Image-GA1100-50

DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

HMS

Product Image-GA1200-50MM

Gibson Assembly® Ultra, Master mixes

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

HMS

Product Image-SYGEGA1100-4X50

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Product Image-K1271

LIC cloning and expression kits, aLICator™, Fermentas

Produsent: Thermo Fisher Scientific
Beskrivelse: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Product Image-GA2100-S

Gibson Assembly® Site directed mutagenesis kit

Artikkenummer: (GA2100-S)
Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Method is a well-established assembly reaction that can be leveraged to join multiple, mutagenised DNA fragments with overlapping ends. Following mutagenesis, DNA fragments of various lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach lends itself to small and large constructs and encompasses both single and multiple insert assemblies.
UOM: 1 * 1 Each

HMS


Product Image-GA1200-50

Gibson Assembly® Ultra kit

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

HMS

Product Image-GA1100-10MM

Gibson Assembly® HiFi 1 Step, Master mixes

Produsent: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

HMS

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T7Select® phage display system, Novagen®

Produsent: Merck Millipore (Novagen)
Beskrivelse: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Product Image-734-2091

Cellekulturplater, 384-brønners, Nunc™

Produsent: Thermo Fisher Scientific
Beskrivelse: PS, behandlet med vevskultur, steril. Ideell for cellekultur, cellefusjon, kloning og virustitreringer.

Product Image-734-2176

Cellekulturplate, Nunc™ Multidish

Produsent: Thermo Fisher Scientific
Beskrivelse: PS, sterile, med lokk. Praktiske for alle celledyrkingsområder, inkludert oppskalering og kloning.

Product Image-734-2080

Cellekulturplate, 96 MicroWell™, Nunclon™∆

Produsent: Thermo Fisher Scientific
Beskrivelse: PS, med høy kant, sterile. Ideelle for celledyrking, kloning, virustitrering og cellefusjon.

Sertifikater

Product Image-E199-100ML

VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology

Produsent: VWR Chemicals
Beskrivelse: Tris (hydroxymethyl) aminomethane.

HMS Sertifikater

Pris på forespørsel
Lager for dette produktet er begrenset, men kan være tilgjengelig i en lagerbygning i nærheten av deg. Vennligst sørg for at du er logget inn på nettstedet, slik at tilgjengelige lager kan vises. Hvis call er fortsatt vises og du trenger hjelp, kan du ringe oss på 1-800-932 - 5000.
Lager for dette punktet er begrenset, men kan være tilgjengelig i en lagerbygning i nærheten av deg. Vennligst sørg for at du er logget inn på nettstedet, slik at tilgjengelige lager kan vises. Hvis call er fortsatt vises og du trenger hjelp, kan du ringe oss på 1-800-932 - 5000.
NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
Dette produktet har blitt blokkert for din organisasjon. Ta kontakt med din innkjøpsavdeling for mer informasjon.
Det opprinnelige produktet er ikke lenger tilgjengelig. Varen som vises er tilgjengelig.
Produkt (er) merket med dette symbolet er ikke lenger tilgjengelig og selges til lageret er tomt. Alternativer kan være tilgjengelige ved å søke med VWR Katalognummer ovenfor. Hvis du trenger ytterligere hjelp, ring VWR kundeservice 22900000
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