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Product Image-BE-404

Experimentation kits, hydrophobic and hydrophilic proteins

Beskrivelse: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.
Artikkenummer: BE-404
UOM: 1 * 1 Each
Produsent: G-Biosciences
Product Image-BE-401

Experimentation kits, physical properties of proteins

Beskrivelse: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.
Artikkenummer: BE-401
UOM: 1 * 1 Each
Produsent: G-Biosciences
Product Image-BE-318

Experimentation kits, nucleic acid quantification

Beskrivelse: The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.
Artikkenummer: BE-318
UOM: 1 * 1 Each
Produsent: G-Biosciences
Product Image-EDVO282

Experimentation kits, principles of enzyme catalysis

Beskrivelse: This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.
Artikkenummer: EDVO282
UOM: 1 * 1 Each
Produsent: EDVOTEK
Product Image-BE-409

Experimentation kits, conservation of genetic information

Beskrivelse: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of three functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.
Artikkenummer: BE-409
UOM: 1 * 1 Each
Produsent: G-Biosciences
Product Image-BE-408

Experimentation kits, protein fingerprinting

Beskrivelse: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of 4 different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.
Artikkenummer: BE-408
UOM: 1 * 1 Each
Produsent: G-Biosciences
Product Image-BE-312

Experimentation kits, expression of a recombinant protein

Beskrivelse: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).
Artikkenummer: BE-312
UOM: 1 * 1 Each
Produsent: G-Biosciences
Product Image-EDVO203

Experimentation kits, isolation of E. coli Chromosomal DNA

Beskrivelse: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.
Artikkenummer: EDVO203
UOM: 1 * 1 Each
Produsent: EDVOTEK
Product Image-EDVOS-47

Linking food science to biotechnology kit

Beskrivelse: Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.
Artikkenummer: EDVOS-47
UOM: 1 * 1 Each
Produsent: EDVOTEK
Product Image-BE-405

Experimentation kits, enzyme analysis

Beskrivelse: This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.
Artikkenummer: BE-405
UOM: 1 * 1 Each
Produsent: G-Biosciences
Product Image-EDVO302

Experimentation kits, purification of the restriction enzyme Eco RI

Beskrivelse: In this experiment, students purify the restriction enzyme, <i>Eco </i>RI. This procedure utilised an ion exchange chromatograph step for <i>Eco </i>RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain <i>Eco </i>RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
Artikkenummer: EDVO302
UOM: 1 * 1 Each
Produsent: EDVOTEK
Product Image-EDVO266

What's in my lunch? quantitative milk allergy ELISA kit

Beskrivelse: Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.
Artikkenummer: EDVO266
UOM: 1 * 1 Each
Produsent: EDVOTEK
Product Image-BE-407

Experimentation kits, protein structure analysis

Beskrivelse: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.
Artikkenummer: BE-407
UOM: 1 * 1 Each
Produsent: G-Biosciences
Product Image-EDVO202

Experimentation kits, Mini-Prep isolation of plasmid DNA

Beskrivelse: Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.
Artikkenummer: EDVO202
UOM: 1 * 1 Each
Produsent: EDVOTEK
Product Image-EDVO235

Experimentation kits, DNA/RNA microarrays

Beskrivelse: Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.
Artikkenummer: EDVO235
UOM: 1 * 1 Each
Produsent: EDVOTEK
Product Image-BE-314

Experimentation kits, mutation detection and analysis

Beskrivelse: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
Artikkenummer: BE-314
UOM: 1 * 1 Each
Produsent: G-Biosciences
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