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Product Image-EDVO115-C

Ready-to-Load™ kits, family pedigree cancer gene detection

Produsent: EDVOTEK
Beskrivelse: In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.

Product Image-BE-403

Experimentation kits: biodiversity study and biomass analysis

Artikkenummer: (BE-403)
Produsent: G-Biosciences
Beskrivelse: Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.
UOM: 1 * 1 Each

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Image Unavailable-ENZOADIKAPCA002D

Anti-CAMK2 Rabbit Polyclonal Antibody

Produsent: ENZO LIFE SCIENCES
Beskrivelse: CaMKII (calmodulin-dependent kinase II) is an enzyme which is activated by increases in intracellular Ca2+ ion concentration and it has been proposed to be pivotal in regulating synaptic strength and maturation of synapses during development.  This process is thought to be critical in memory and learning and in establishing the specificity of synaptic connections. There are over two dozen alternative splice variants of CaMKII which are encoded by four genes, a, b, g, and d with apparent molecular masses of 50-60 kDa.  CaMKII is widely distributed in many tissues, but is highly expressed in brain. Several studies demonstrate that CaMKII is a multifunctional enzyme which modulates the synaptic strength by binding to a subunit of NMDA receptors and promoting the phosphorylation of this NMDA receptor subunit.  CaMKII also regulates DLG localization at synapses by co-localization with DLG in the same protein complex.  Experimental data suggest that CaMKII is critically involved in the development of morphine tolerance as well as dependence and inhibition of this enzyme may have some therapeutic benefit in the treatment of opiate tolerance and dependence.  It also has been demonstrated that d CaMKII isozyme is down-regulated in human tumor cells indicating a role for d CaMKII isozymes in cellular differentiation.  Changes in d CaMKII isozyme expression pattern in human hearts during heart failure suggest that CaMKII is important for regulation of heart function.  This immunoaffinity purified antibody detects proteins of 50-60 kDa, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, hamster, guinea pig, chicken and rabbit origins. Recombinant rat calmodulin-dependent protein kinase II a subunits expressed in sf9 insect cells are also detected. As the sequences of the rat a, d, and g isoforms are conserved over this amino terminus region, this antibody is expected to recognize the d, g and a isoforms. This antibody is not expected to cross-react with the b isoform. Proteins of unknown identity, ~40, 45 and 90 kDa, may be detected on immunoblot analysis with some lysates.

New Product

Product Image-BE-407

Experimentation kits, protein structure analysis

Artikkenummer: (BE-407)
Produsent: G-Biosciences
Beskrivelse: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.
UOM: 1 * 1 Each
Product Image-BE-501

Experimentation kits, antigen-antibody interactions

Artikkenummer: (BE-501)
Produsent: G-Biosciences
Beskrivelse: This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.
UOM: 1 * 1 Each
Product Image-EDVO315

Kits: in search of the sickle cell gene by southern blot, Ready-to-Load™

Artikkenummer: (EDVO315)
Produsent: EDVOTEK
Beskrivelse: Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.
UOM: 1 * 1 Items

HMS


Product Image-EDVO153

Experimentation kits, determination of protein molecular weight (polyacrylamide-based)

Artikkenummer: (EDVO153)
Produsent: EDVOTEK
Beskrivelse: Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.
UOM: 1 * 1 Each

HMS


Product Image-BE-314

Experimentation kits, mutation detection and analysis

Artikkenummer: (BE-314)
Produsent: G-Biosciences
Beskrivelse: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
UOM: 1 * 1 Each
Product Image-EDVO202

Experimentation kits, Mini-Prep isolation of plasmid DNA

Artikkenummer: (EDVO202)
Produsent: EDVOTEK
Beskrivelse: Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.
UOM: 1 * 1 Each
Product Image-EDVO951

Experimentation kits, water quality testing I: chromogenic analysis of water bacteria contaminants

Artikkenummer: (EDVO951)
Produsent: EDVOTEK
Beskrivelse: Safe drinking water is vitally important to health. Both pathogenic and harmless bacteria can be found in the guts of mammals and birds. Testing water for every possible type of pathogenic bacteria is slow and costly. Thus, water is tested for a characteristic type of gut bacteria - the coliforms - including the familiar <i>E. coli</i>. Presence of coliforms is an indicator for faecal contamination. In this experiment, students test for coliforms in simulated contaminated water using colour and fluorescent reagents. They can use these same reagents to test water samples from the environment. As an extension activity, a Gram Stain test can be performed on the collected samples.
UOM: 1 * 1 Each

HMS


Product Image-BE-406

Eksperimentsett, proteinelektroforese

Artikkenummer: (BE-406)
Produsent: G-Biosciences
Beskrivelse: Ved å bruke proteinelektroforesesettet vil studentene lære prinsippene for ulike typer elektroforese, inkludert denaturerende og ikke-denaturerende elektroforese, og hvordan denne kraftige teknikken brukes til å analysere proteiner. Settet vil introdusere elevene til de forskjellige separasjonsmatrisene som for tiden er i bruk og vil forstå deres forskjellige separasjonsegenskaper og deres rolle i proteinanalyse. Studentene har muligheten til å støpe sine egne elektroforesegeler ved hjelp av polyakrylamid eller bruke forhåndsstøpte kommersielt tilgjengelige geler. Dette settet leveres med alle reagenser, buffere og forsyninger som trengs for å støpe akrylamidgeler, forberede proteinprøver, kjøre elektroforese og farge gelene for visualisering av proteinbånd. Testproteinprøver og proteinstandarder er også gitt.
UOM: 1 * 1 Each
Product Image-BE-404

Experimentation kits, hydrophobic and hydrophilic proteins

Artikkenummer: (BE-404)
Produsent: G-Biosciences
Beskrivelse: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.
UOM: 1 * 1 Each
Product Image-201-0216

Capillary tube on foot

Artikkenummer: (201-0216)
Produsent: GLASWARENFABRIK KARL HECHT
Beskrivelse: Five communicating glass tubes with different inner diameters. Used to show capillary forces and how they are affected by the inner diameter of the pipes.
UOM: 1 * 1 Items
Product Image-765-0969

Communicating tubes

Artikkenummer: (765-0969)
Produsent: GLASWARENFABRIK KARL HECHT
Beskrivelse: Five glass tubes of different shape and inner dimensions. Down to each other. When filling the liquid in the pipes, it is clear that the height of the stowage in the tubes. pipes are independent of the design of the pipes. Comes with foot.
UOM: 1 * 1 Items
Image Unavailable-BOSSBS-5846R-CY5.5

Anti-ADAM11 Rabbit Polyclonal Antibody (Cy5.5®)

Artikkenummer: (BOSSBS-5846R-CY5.5)
Produsent: Bioss
Beskrivelse: ADAM11 was first described as MDC (Metalloproteinase-like disintergin-like cysteine-rich protein) from analysis of human brain libraries, in search of brain-specific proteins. Two splice variants with different carboxyterminal ends were described. The message was found only in the brain in this publication. Another group identified ADAM11 in the human brain, where ADAM11 was thought to be involved in cell migration and spatial patterning. ADAM11 was mapped to 17q21.3, a region of interest for breast cancer, and mutations in ADAM11 are associated with some breast cancers. Retinoic acid caused a doubling in ADAM11 message levels over 24 hours in NT2/D1 cells, a human embryonic carcinoma cell line. ADAM11 null mutant mice have deficits in spatial learning and motor coordination, although they did have normal cell migration and differentiation during development. ADAM11 is a member of the ADAMs family (A Disintegrin And Metalloproteinase), but does not contain the canonical HExxHxxxxH zinc-binding metalloproteinase catalytic site. The domain structure of the full-length ADAM11 includes a signal sequence, propeptide domain, metalloproteinase-like domain, disintegrin-like domain, cys-rich domain, EGF-like domain, a spacer region, then the transmembrane domain and a short cytoplasmic domain.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-5846R-A750

Anti-ADAM11 Rabbit Polyclonal Antibody (Alexa Fluor® 750)

Artikkenummer: (BOSSBS-5846R-A750)
Produsent: Bioss
Beskrivelse: ADAM11 was first described as MDC (Metalloproteinase-like disintergin-like cysteine-rich protein) from analysis of human brain libraries, in search of brain-specific proteins. Two splice variants with different carboxyterminal ends were described. The message was found only in the brain in this publication. Another group identified ADAM11 in the human brain, where ADAM11 was thought to be involved in cell migration and spatial patterning. ADAM11 was mapped to 17q21.3, a region of interest for breast cancer, and mutations in ADAM11 are associated with some breast cancers. Retinoic acid caused a doubling in ADAM11 message levels over 24 hours in NT2/D1 cells, a human embryonic carcinoma cell line. ADAM11 null mutant mice have deficits in spatial learning and motor coordination, although they did have normal cell migration and differentiation during development. ADAM11 is a member of the ADAMs family (A Disintegrin And Metalloproteinase), but does not contain the canonical HExxHxxxxH zinc-binding metalloproteinase catalytic site. The domain structure of the full-length ADAM11 includes a signal sequence, propeptide domain, metalloproteinase-like domain, disintegrin-like domain, cys-rich domain, EGF-like domain, a spacer region, then the transmembrane domain and a short cytoplasmic domain.
UOM: 1 * 100 µl
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Lager for dette punktet er begrenset, men kan være tilgjengelig i en lagerbygning i nærheten av deg. Vennligst sørg for at du er logget inn på nettstedet, slik at tilgjengelige lager kan vises. Hvis call er fortsatt vises og du trenger hjelp, kan du ringe oss på 1-800-932 - 5000.
NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
NB Dette produkt er regulert i henhold til norsk lov.
Dersom ytterligere informasjon er nødvendig fra deg vil du bli kontaktet av VWR via e-post.
OBS! Etanol er underlagt bestemmelse om særavgift, jfr LOV-1993-05-19-11-§1. VWR selger kun etanol til kunder som kan fremlegge avgiftsfritak for udenaturert etanol, jfr. § 3-3-7 ( forskrifter om særavgifter).
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