Produsent: Thermo Fisher Scientific

Beskrivelse: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Produsent: Thermo Fisher Scientific

Beskrivelse: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Produsent: Thermo Fisher Scientific

Beskrivelse: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Produsent: Thermo Fisher Scientific

Beskrivelse: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Artikkenummer: (K1441)

Produsent: Thermo Fisher Scientific

Beskrivelse: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

UOM: 1 * 1 Each

Tilbud

Produsent: Thermo Fisher Scientific

Beskrivelse: GeneJET™ Whole Blood Genomic DNA Purification Mini Kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Produsent: Thermo Fisher Scientific

Beskrivelse: GeneJET™ Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from a wide variety of plant species and tissue types.

Artikkenummer: (SM1103)

Produsent: Thermo Fisher Scientific

Beskrivelse: FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

UOM: 1 * 1 Each

Tilbud

Artikkenummer: (SM0383)

Produsent: Thermo Fisher Scientific

Beskrivelse: MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.

UOM: 1 * 1 Each

Tilbud

Artikkenummer: (ER1751)

Produsent: Thermo Fisher Scientific

Beskrivelse: CCNGGA sites and cuts best at 37 °C in Tango buffer.

UOM: 1 * 1 Each

Tilbud

Produsent: Thermo Fisher Scientific

Beskrivelse: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

Artikkenummer: (EN0525)

Produsent: Thermo Fisher Scientific

Beskrivelse: DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyses phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'- phosphate and 3'- OH groups.

UOM: 1 * 1 Each

HMS Tilbud

Produsent: Thermo Fisher Scientific

Beskrivelse: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Artikkenummer: (R0861)

Produsent: Thermo Fisher Scientific

Beskrivelse: Cleland's reagens

UOM: 1 * 5 g

HMS Sertifikater Tilbud

Artikkenummer: (SM1821)

Produsent: Thermo Fisher Scientific

Beskrivelse: RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.

UOM: 1 * 1 Each

Tilbud

Produsent: Thermo Fisher Scientific

Beskrivelse: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.